[摘要]

 

      目的：研究神經(jīng)生長因子(Nerve growth factor, NGF)對人血管內(nèi)皮細(xì)胞(Human vascular endothelial cell, HEVC)增殖作用的影響。

      方法：將NGF作用于體外培養(yǎng)的HEVC304，利用酶聯(lián)免疫反應(yīng)檢測其增殖率，利用免疫組織化學(xué)的方法檢測對增殖細(xì)胞核抗原(Proliferating cell nuclear antigen, PCNA)的表達(dá)和標(biāo)記指數(shù)。

      結(jié)果：NGF作用組較對照組其細(xì)胞增殖率明顯增加(P<0.001), NGF中和抗體組其細(xì)胞增殖率下降明顯(P<0.05)。NGF作用組較對照組能明顯增加HEVC對PCNA的表達(dá)和PCNA標(biāo)記指數(shù)（P<0.05）， NGF中和抗體組其PCNA表達(dá)和PCNA標(biāo)記指數(shù)明顯減少（P<0.05）。

      結(jié)論：NGF能明顯促進(jìn)HEVC的增殖。
　


　　　　Study of effect of nerve growth factor on the human vascular endothelial cell proliferation

　　　Abstract：   Objective   To investigate the effect of nerve growth factor（NGF）on human vascular endothelial cell.  Methods   The effect of NGF on human umbilica vein vascular endothelial cell were used in the third passage.Endothelial cell proliferative rate was assessed using the enzyme linked immunoassay.The proliferating cell nuclear antigen expression and  proliferating cell nuclear antigen lable index were assessed using the immunnohischemistry.  Results  Compared with the control group，the NGF groups whose endothelial cell proliferative rate were increased significantly（P<0.001）.NGF neutralizing antibody de creased the mitogenic effect of NGF significantly（P<0.05）.NGF also increased PCNA expression and PCNA lable index on HEVC304 .NGF－neutralizing antibody decreaed PCNA expression on NGF－stimulated HEVC304（P<0.05）.  Conclusion  NGF could significantly induced the mitosis of human umbilica vein vascular endothelial cell.